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Pi-minimal Screen CS-127

Pi-PEG Screen CS-128

Pi-minimal Screen HTS CS-211L

Pi-PEG Screen HTS CS-212L

The Pi-Screens were developed at the MRC Laboratory of Molecular Biology (Cambridge, UK) for efficient crystallization screening of soluble proteins (Pi-minimal Screen) and integral membrane proteins (Pi-PEG Screen). The approach is based on incomplete factorial design.

The unique formulation was generated following a strategy named Pi sampling [1] in order to create novel combinations of precipitants, buffers and additives across a standard 96-condition plate layout. Thus, the diversity amongst the crystallization conditions is ideal for initial screening.

The Pi-minimal Screen includes 36 components, i.e. 12 precipitants, 12 buffers systems and 12 salts. Buffers employed in the Pi-minimal screen are buffer systems (acid-base pairs, e.g. HEPES and HEPES sodium salt). Consequently, pH can be adjusted by mixing 2 stock solutions at different ratios during later optimizations.
The efficiency of the Pi-minimal Screen was demonstrated by the crystallization of 10 proteins before its commercialization [1].

The Pi-PEG Screen includes various polyethylene glycol mixtures, additives and buffers covering a pH range from 4,0 – 9,5 and hence is suitable for integral membrane proteins as well as for soluble proteins.
The efficiency of the Pi-PEG screen was demonstrated by the crystallization of a G-protein coupled receptor (GPCR) when quality crystals could not be produced with other commercially available screens.

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